Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-activity relationship of Cu(II) and Pd(II) thiosemicarbazone complexes with anticancer and antibacterial properties

doi: 10.1080/14756366.2026.2742455

Figure Lengend Snippet: IC 50 values determined for the selected complexes.

Article Snippet: Three human malignant melanoma cell lines, A-375 (CRL-1619 , American Type Culture Collection, Manassas, VA, USA), SK-MEL-28 (HTB-71 , American Type Culture Collection, Manassas, VA, USA), and G-361 (CRL-1424 , American Type Culture Collection, Manassas, VA, USA), were used in this study.

Techniques:

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-activity relationship of Cu(II) and Pd(II) thiosemicarbazone complexes with anticancer and antibacterial properties

doi: 10.1080/14756366.2026.2742455

Figure Lengend Snippet: The results of mRNA gene expression analysis in SK-MEL-28 and A-375 cells treated with Cu(b-TSC) 2 , and in G-361 cells treated with Pd(dm-TSC-pCl) 2 for 48 h at concentrations corresponding to the IC 50 value. The results are expressed as relative quantification (RQ) values and presented as means. The mean RQ values are illustrated using a colour scale. The control was considered the representative reference sample for relative gene expression analysis. B-ACT and RNA18SN5 were selected as reference genes.

Article Snippet: Three human malignant melanoma cell lines, A-375 (CRL-1619 , American Type Culture Collection, Manassas, VA, USA), SK-MEL-28 (HTB-71 , American Type Culture Collection, Manassas, VA, USA), and G-361 (CRL-1424 , American Type Culture Collection, Manassas, VA, USA), were used in this study.

Techniques: Gene Expression, Analysis, Quantitative Proteomics, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-activity relationship of Cu(II) and Pd(II) thiosemicarbazone complexes with anticancer and antibacterial properties

doi: 10.1080/14756366.2026.2742455

Figure Lengend Snippet: Cell cycle analysis of melanoma cells following treatment with selected complexes, compared to untreated controls. ( A ) Quantitative distribution of cell cycle phases (SubG1, G1, S, and G2/M) in A-375 and SK-MEL-28 cells treated with Cu(b-TSC) 2 , and G-361 cells treated with Pd(dm-TSC-pCl) 2 at their respective IC 50 concentrations. ( B ) Representative DNA content histograms for each condition, illustrating the distribution of cell populations in SubG1 (M1), G1 (M2), S (M3), and G2/M (M4) phases; the SubG1 fraction reflects DNA fragmentation associated with apoptosis.

Article Snippet: Three human malignant melanoma cell lines, A-375 (CRL-1619 , American Type Culture Collection, Manassas, VA, USA), SK-MEL-28 (HTB-71 , American Type Culture Collection, Manassas, VA, USA), and G-361 (CRL-1424 , American Type Culture Collection, Manassas, VA, USA), were used in this study.

Techniques: Cell Cycle Assay

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-activity relationship of Cu(II) and Pd(II) thiosemicarbazone complexes with anticancer and antibacterial properties

doi: 10.1080/14756366.2026.2742455

Figure Lengend Snippet: Apoptosis analysis of melanoma cells following treatment with selected complexes, compared to untreated controls. ( A ) Quantitative assessment of A-375 and SK-MEL-28 cells treated with Cu(b-TSC) 2 , and G-361 cells treated with Pd(dm-TSC-pCl) 2 at their respective IC 50 concentrations. Cells were stained with Annexin V-CF488A, Hoechst 33342, and propidium iodide (PI) and analysed using the NucleoCounter ® NC-3000 ™ image cytometer. The bar graph represents the percentage of live (Annexin V-negative/PI-negative), early apoptotic (Annexin V-positive/PI-negative), late apoptotic (Annexin V-positive/PI-positive), and necrotic cells. (B) Representative scatter plots for each condition, illustrating the distribution of cell populations divided into four quadrants: lower left (Q1ll), viable cells; lower right (Q1lr), early apoptotic cells; upper right (Q1ur), late apoptotic cells; and upper left (Q1ul), necrotic cells.

Article Snippet: Three human malignant melanoma cell lines, A-375 (CRL-1619 , American Type Culture Collection, Manassas, VA, USA), SK-MEL-28 (HTB-71 , American Type Culture Collection, Manassas, VA, USA), and G-361 (CRL-1424 , American Type Culture Collection, Manassas, VA, USA), were used in this study.

Techniques: Analysis, Staining, Cytometry

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-activity relationship of Cu(II) and Pd(II) thiosemicarbazone complexes with anticancer and antibacterial properties

doi: 10.1080/14756366.2026.2742455

Figure Lengend Snippet: IC 50 values determined for Cu(b-TSC) 2 and vemurafenib on parental and vemurafenib-resistant SK-MEL-28 and A-375 cells.

Article Snippet: Three human malignant melanoma cell lines, A-375 (CRL-1619 , American Type Culture Collection, Manassas, VA, USA), SK-MEL-28 (HTB-71 , American Type Culture Collection, Manassas, VA, USA), and G-361 (CRL-1424 , American Type Culture Collection, Manassas, VA, USA), were used in this study.

Techniques:

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-activity relationship of Cu(II) and Pd(II) thiosemicarbazone complexes with anticancer and antibacterial properties

doi: 10.1080/14756366.2026.2742455

Figure Lengend Snippet: Exploratory plots of log(IC 50 ) against the calculated logP o/w , qPSA NOS /SASA, logS, and E solv descriptors for the investigated Cu(II) complexes. Linear regression lines and the corresponding coefficients of determination (R 2 ) are shown.

Article Snippet: Three human malignant melanoma cell lines, A-375 (CRL-1619 , American Type Culture Collection, Manassas, VA, USA), SK-MEL-28 (HTB-71 , American Type Culture Collection, Manassas, VA, USA), and G-361 (CRL-1424 , American Type Culture Collection, Manassas, VA, USA), were used in this study.

Techniques:

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-activity relationship of Cu(II) and Pd(II) thiosemicarbazone complexes with anticancer and antibacterial properties

doi: 10.1080/14756366.2026.2742455

Figure Lengend Snippet: Exploratory plots of log(IC 50 ) against the calculated logP o/w , qPSA NOS /SASA, logS, and E solv descriptors for the investigated Pd(II) complexes. Linear regression lines and the corresponding coefficients of determination (R 2 ) are shown.

Article Snippet: Three human malignant melanoma cell lines, A-375 (CRL-1619 , American Type Culture Collection, Manassas, VA, USA), SK-MEL-28 (HTB-71 , American Type Culture Collection, Manassas, VA, USA), and G-361 (CRL-1424 , American Type Culture Collection, Manassas, VA, USA), were used in this study.

Techniques:

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Structure-activity relationship of Cu(II) and Pd(II) thiosemicarbazone complexes with anticancer and antibacterial properties

doi: 10.1080/14756366.2026.2742455

Figure Lengend Snippet: Cell viability of SK-MEL-28 cancer cells after 48 h of incubation with the studied compounds at 10 µM. The three sections represent 3,4-dimethoxybenzylidene-, 3,4-dichlorobenzylidene-, and 4-bromobenzylidene- derivatives, respectively. Green, blue, and orange bars represent free organic ligands, Cu(II) complexes, and Pd(II) complexes, respectively.

Article Snippet: Three human malignant melanoma cell lines, A-375 (CRL-1619 , American Type Culture Collection, Manassas, VA, USA), SK-MEL-28 (HTB-71 , American Type Culture Collection, Manassas, VA, USA), and G-361 (CRL-1424 , American Type Culture Collection, Manassas, VA, USA), were used in this study.

Techniques: Incubation